Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: Effects of AuNPs + radiation combined treatment on the cell proliferation of normal hepatocytes and hepatic stellate cell line. (A) MTT assays were performed using HH cells treated with AuNPs or X-ray irradiation. (B) Transmission electron microscopy images of AuNPs uptake inside the cell. (C) MTT assays were performed using hepatocyte cells (HH: left panel) and hepatic stellate cell line (LX-2: right panel) treated with AuNPs or X-ray irradiation, or a combination of the two treatments. The blue arrows indicate the magnification of AuNPs. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; HH, human hepatocyte cell.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Irradiation, Transmission Assay, Electron Microscopy

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: Cell SF of AuNPs + radiation combined treatment of normal hepatocytes. Clonogenic assay of hepatocytes after treatment with AuNPs or X-ray irradiation or a combination of the two treatments. SF, survival fraction; IR, irradiation.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Clonogenic Assay, Irradiation

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: Effects on apoptosis of AuNPs + radiation combined treatment of hepatocytes. (A) Cells were treated with PI at 48 h post-treatment after each indicated treatment and analyzed by FACS. (B) The indicated antibodies were used for western blotting of hepatocytes treated with AuNPs or X-ray irradiation or a combination of the two treatments. Values represent the means of 3 experiments ± SD. (C) Cells were treated with AuNPs and/or X-ray irradiation or a combination of the two treatments for 24 h. The cell-cycle distribution of subG1 was analyzed quantitatively. *, P<0.05 and **, P<0.01. IR, irradiation; PARP, poly (ADP-ribose) polymerase; PI, propidium iodide; FACS, fluorescence-activated cell sorting.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Western Blot, Irradiation, Fluorescence, FACS

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: IL-8 concentrations secreted from normal hepatocytes after AuNPs + X-ray irradiation combined treatment. In all therapeutic groups, a decrease in the amount of IL-8 was observed in the radiation alone group. The combination therapy did not lead to significantly lower levels of IL-8 than radiation alone. IL-8, interleukin-8; IR, irradiation.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Irradiation

Journal: Oncotarget

Article Title: Inflammatory and oncogenic roles of a tumor stem cell marker doublecortin-like kinase (DCLK1) in virus-induced chronic liver diseases

doi:

Figure Lengend Snippet: A. Hepatocytes preparations were made from adult human healthy liver and cultured in Matrigel for 3-6 weeks. Induction of perinuclear DCLK1 expression in normal human hepatocytes (NHHs) within the spheroids and their differentiation into multiple cell-lineages are shown by immunohistochemical staining of the spheroids for DCLK1 and α-fetoprotein/cytokeratin 19 (AFP/CK19) co-expression or cytoplasmic CK19 (both mark stem-like progenitor cells). B. Human serum (5%)-induced differentiation and morphological changes in the NHH-spheroids (bright-field images). Representative morphological patterns for differentiated (e.g. neurons, mesenchymal cells) or serum-resistant (extreme right panel) of the spheroids are shown.

Article Snippet: The cryopreserved normal human hepatocytes (NHHs) were purchased from BD Biosciences.

Techniques: Cell Culture, Expressing, Immunohistochemical staining, Staining

Journal: Molecular Medicine Reports

Article Title: Paeonol alleviates lipopolysaccharide-induced hepatocytes injury through alteration of mitochondrial function and NF-κB translocation

doi: 10.3892/mmr.2021.12419

Figure Lengend Snippet: Gel electrophoresis separation of PCR products. (A-D) PCR products of genomic DNA samples by using HeLa gDNA detection primer set. (E-H) products of genomic DNA samples by using Human gDNA control primer set. (A and E) HeLa positive control PCR template, (B and F) HeLa cells, (C and G) L02 cells mixed with 1% HeLa cells and (D and H) L02 cells. HLD, HeLa gDNA detection primer set; HGC, Human gDNA control primer set.

Article Snippet: L02 human normal hepatocytes were purchased from BioVector NTCC Inc. and the cells were cultured in a 5% CO 2 atmosphere at 37°C.

Techniques: Nucleic Acid Electrophoresis, Control, Positive Control

Journal: Molecular Medicine Reports

Article Title: Paeonol alleviates lipopolysaccharide-induced hepatocytes injury through alteration of mitochondrial function and NF-κB translocation

doi: 10.3892/mmr.2021.12419

Figure Lengend Snippet: PAE increases LPS decreased cell viability of L02 cells. (A) The injury effect of LPS on cell viability of L02 cells. Cells were incubated with different concentration of LPS (0.05, 0.5, 5.0 or 10.0 µg/ml) for 12 h. (B) The effect of PAE alone on cell viability of L02 cells. Cells were incubated with different concentration of PAE (0.1, 1.0, 10.0 or 100.0 µg/ml) for 12 h. (C) The protective effect of PAE on LPS damaged L02 cells. Cells were incubated with different concentration of PAE (0.1, 1.0 or 10.0 µg/ml) prior 1 h and then co-incubated with LPS (10 µg/ml) for 12 h. Cell viability was assessed by CCK-8. Data represent mean ± standard error of the mean of six independent experiments. *P<0.05 or **P<0.01 vs. control or LPS. PAE, paeonol; LPS, lipopolysaccharide.

Article Snippet: L02 human normal hepatocytes were purchased from BioVector NTCC Inc. and the cells were cultured in a 5% CO 2 atmosphere at 37°C.

Techniques: Incubation, Concentration Assay, CCK-8 Assay, Control

Journal: Molecular Medicine Reports

Article Title: Paeonol alleviates lipopolysaccharide-induced hepatocytes injury through alteration of mitochondrial function and NF-κB translocation

doi: 10.3892/mmr.2021.12419

Figure Lengend Snippet: PAE inhibits LPS increased ALT and AST of L02 cells. Cells were incubated with LPS at 10.0 µg/ml for 12 h or cells were incubated with PAE at 10.0 µg/ml prior 1 h and then co-incubated with LPS (10 µg/ml) for 12 h. The activities of (A) ALT and (B) AST were assayed according to the manufacturer's instructions. Data represent mean ± standard error of the mean of six independent experiments. *P<0.05 or **P<0.01 vs. control. PAE, paeonol; LPS, lipopolysaccharide; ALT, alanine aminotransferase; AST, aspartate transaminase.

Article Snippet: L02 human normal hepatocytes were purchased from BioVector NTCC Inc. and the cells were cultured in a 5% CO 2 atmosphere at 37°C.

Techniques: Incubation, Control

Journal: Molecular Medicine Reports

Article Title: Paeonol alleviates lipopolysaccharide-induced hepatocytes injury through alteration of mitochondrial function and NF-κB translocation

doi: 10.3892/mmr.2021.12419

Figure Lengend Snippet: PAE inhibits LPS induced the apoptosis of L02 cells. Cells were incubated with LPS at 10.0 µg/ml for 12 h or cells were incubated with PAE at 10.0 µg/ml prior 1 h and then co-incubated with LPS (10 µg/ml) for 12 h. Cell apoptosis was observed with laser confocal microscopy (scale bar=75 µm). The population was separated into three groups: Live cells with a low level of fluorescence, apoptotic cells in the earlier period with green fluorescence and necrotic and advanced stage apoptotic cells with both red and green fluorescence. PAE, paeonol; LPS, lipopolysaccharide.

Article Snippet: L02 human normal hepatocytes were purchased from BioVector NTCC Inc. and the cells were cultured in a 5% CO 2 atmosphere at 37°C.

Techniques: Incubation, Confocal Microscopy, Fluorescence

Journal: Molecular Medicine Reports

Article Title: Paeonol alleviates lipopolysaccharide-induced hepatocytes injury through alteration of mitochondrial function and NF-κB translocation

doi: 10.3892/mmr.2021.12419

Figure Lengend Snippet: PAE decreases LPS induced ROS generation of L02 cells (bar=75µm). Cells were incubated with LPS at 10.0 µg/ml for 12 h or cells were incubated with PAE at 10.0 µg/ml prior 1 h and then co-incubated with LPS (10 mg/ml) for 4 h. Intracellular ROS was observed with laser confocal microscopy. The population was separated into two groups: Left panels were cells observed by phase contrast and right panels were cells observed by fluorescence microscopy. PAE, paeonol; LPS, lipopolysaccharide; ROS, reactive oxygen species; DCF, dichlorofluorescein.

Article Snippet: L02 human normal hepatocytes were purchased from BioVector NTCC Inc. and the cells were cultured in a 5% CO 2 atmosphere at 37°C.

Techniques: Incubation, Confocal Microscopy, Fluorescence, Microscopy

Journal: Molecular Medicine Reports

Article Title: Paeonol alleviates lipopolysaccharide-induced hepatocytes injury through alteration of mitochondrial function and NF-κB translocation

doi: 10.3892/mmr.2021.12419

Figure Lengend Snippet: PAE inhibits LPS increased MDA and increases LPS decreased SOD level of L02 cells. Cells were incubated with LPS at 10.0 µg/ml for 12 h or cells were incubated with PAE at 10.0 µg/ml prior 1 h and then co-incubated with LPS (10 µg/ml) for 12 h. (A) MDA and (B) SOD were assayed by respective kits. Data represent mean ± standard error of the mean of six independent experiments. *P<0.05 or **P<0.01 vs. control. PAE, paeonol; LPS, lipopolysaccharide; MDA, malondialdehyde; SOD, superoxide dismutase.

Article Snippet: L02 human normal hepatocytes were purchased from BioVector NTCC Inc. and the cells were cultured in a 5% CO 2 atmosphere at 37°C.

Techniques: Incubation, Control

Journal: Molecular Medicine Reports

Article Title: Paeonol alleviates lipopolysaccharide-induced hepatocytes injury through alteration of mitochondrial function and NF-κB translocation

doi: 10.3892/mmr.2021.12419

Figure Lengend Snippet: PAE increases LPS-decreased mitochondrial transmembrane potential in L02 cells (bar=75 µm). Cells were incubated with LPS at 10.0 µg/ml for 12 h or cells were incubated with PAE at 10.0 µg/ml prior 1 h and then co-incubated with LPS (µg/ml) for 12 h. The mitochondrial membrane potential was measured by laser confocal microscopy. The population was separated into four groups: Left panels were cells observed by bright light and right panels were the merged field of red (for JC-1 aggregates) and green color (for JC-1 monomers). PAE, paeonol; LPS, lipopolysaccharide.

Article Snippet: L02 human normal hepatocytes were purchased from BioVector NTCC Inc. and the cells were cultured in a 5% CO 2 atmosphere at 37°C.

Techniques: Incubation, Membrane, Confocal Microscopy

Journal: Molecular Medicine Reports

Article Title: Paeonol alleviates lipopolysaccharide-induced hepatocytes injury through alteration of mitochondrial function and NF-κB translocation

doi: 10.3892/mmr.2021.12419

Figure Lengend Snippet: PAE inhibits LPS-promoted nuclear translocation of NF-κB (scale bar=75 µm). Cells were incubated with LPS at 10.0 µg/ml for 12 h or cells were incubated with PAE at 10.0 µg/ml prior 1 h and then co-incubated with LPS (10 µg/ml) for 12 h. The NF-κB level in the nuclei of L02 cells was detected by immunofluorescence staining. The right panels were the merged field of red (for NF-κB subunit p 65) and blue color (for cell nucleus). PAE, paeonol; LPS, lipopolysaccharide.

Article Snippet: L02 human normal hepatocytes were purchased from BioVector NTCC Inc. and the cells were cultured in a 5% CO 2 atmosphere at 37°C.

Techniques: Translocation Assay, Incubation, Immunofluorescence, Staining

Journal: Bioengineered

Article Title: Long non-coding RNA AC012668 suppresses non-alcoholic fatty liver disease by competing for microRNA miR-380-5p with lipoprotein-related protein LRP2

doi: 10.1080/21655979.2021.1960463

Figure Lengend Snippet: NAFLD models established to investigate changes in AC012668 expression. (a) Heat map of differentially expressed lncRNAs in NAFLD. (b) Cell viability and (c and d) apoptosis of LO2 cells treated with FFA. (e and f) Oil-Red O staining image and TG level in the liver tissues of HFD mice. (g) AC012668 expression levels in the liver tissues of HFD mice. (h) Expression of AC012668 in FFA-treated cells. *P < 0.05 vs. control; **P < 0.01 vs. control

Article Snippet: Normal human hepatocyte lines LO2 were purchased from Biowit Biotechnology Inc. (cat no. C0009).

Techniques: Expressing, Staining

Journal: Bioengineered

Article Title: Long non-coding RNA AC012668 suppresses non-alcoholic fatty liver disease by competing for microRNA miR-380-5p with lipoprotein-related protein LRP2

doi: 10.1080/21655979.2021.1960463

Figure Lengend Snippet: AC012668 suppresses TG/lipid accumulation and lipogenesis in LO2 cells. (a) AC012668 expression level in FFA-treated LO2 cells transfected with the AC012668 overexpression plasmid. (b) mRNA and (c and d) protein expression levels of SCD1, SREBP1 , and FAS in FFA-treated LO2 cells. (e) TG level and (f) Oil-Red O staining in FFA-treated LO2 cells. *P < 0.05 vs. pcDNA3.1 or AC012668.

Article Snippet: Normal human hepatocyte lines LO2 were purchased from Biowit Biotechnology Inc. (cat no. C0009).

Techniques: Expressing, Transfection, Over Expression, Plasmid Preparation, Staining

Journal: Bioengineered

Article Title: Long non-coding RNA AC012668 suppresses non-alcoholic fatty liver disease by competing for microRNA miR-380-5p with lipoprotein-related protein LRP2

doi: 10.1080/21655979.2021.1960463

Figure Lengend Snippet: miR-380-5p directly targets AC012668 . (a) Wild and mutant types of AC012668 reporters labeled with luciferase. (b) Expression levels of miR-380-5p in AC012668 -enhanced/-inhibited LO2 cells. (c) Relative luciferase activities in wild-type and mutant AC012668 groups compared with the mimic negative control group. (d) Relative enrichment of AC012668 in the biotinylated miR-380-5p group. (e) AC012668 expression levels in the liver tissues of HFD mice. (f) Expression level of miR-380-5p in LO2 cells treated with 1 mM FFA. *P < 0.05 vs. biotin-NC; **P < 0.01 vs. si-NC, vector, mimic NC, or control

Article Snippet: Normal human hepatocyte lines LO2 were purchased from Biowit Biotechnology Inc. (cat no. C0009).

Techniques: Mutagenesis, Labeling, Luciferase, Expressing, Negative Control, Plasmid Preparation

Journal: Bioengineered

Article Title: Long non-coding RNA AC012668 suppresses non-alcoholic fatty liver disease by competing for microRNA miR-380-5p with lipoprotein-related protein LRP2

doi: 10.1080/21655979.2021.1960463

Figure Lengend Snippet: miR-380-5p promotes TG/lipid accumulation and lipogenesis in LO2 cells transfected with AC012668 . (a) AC012668 expression detected using reverse transcription-quantitative polymerase chain reaction. (b) mRNA and (c and d) protein levels of SCD1, SREBP1 , and FAS . (e) TG level and (f) lipid deposition. *P < 0.05 vs. AC012668 + mimic NC; **P < 0.01 vs. vector

Article Snippet: Normal human hepatocyte lines LO2 were purchased from Biowit Biotechnology Inc. (cat no. C0009).

Techniques: Transfection, Expressing, Real-time Polymerase Chain Reaction, Plasmid Preparation

Journal: Bioengineered

Article Title: Long non-coding RNA AC012668 suppresses non-alcoholic fatty liver disease by competing for microRNA miR-380-5p with lipoprotein-related protein LRP2

doi: 10.1080/21655979.2021.1960463

Figure Lengend Snippet: LRP2 is a target of miR-380-5p . (a) Wild and mutant types of LRP2 reporters labeled with luciferase. (b) mRNA expression of LRP2 in FFA-treated LO2 cells transfected with the miR-380-5p mimic or inhibitor. (c) Relative luciferase activities in wild-type and mutant LRP2 groups compared with the mimic negative control group. (d) Relative enrichment of LRP2 in the biotinylated miR-380-5p group. (e) AC012668 expression levels in the liver tissues of HFD mice. (f) Expression level of LRP2 in FFA-treated LO2 cells. *P < 0.05 vs. biotin-NC; **P < 0.01 vs. inhibitor NC, mimic NC, or control

Article Snippet: Normal human hepatocyte lines LO2 were purchased from Biowit Biotechnology Inc. (cat no. C0009).

Techniques: Mutagenesis, Labeling, Luciferase, Expressing, Transfection, Negative Control

Journal: Bioengineered

Article Title: Long non-coding RNA AC012668 suppresses non-alcoholic fatty liver disease by competing for microRNA miR-380-5p with lipoprotein-related protein LRP2

doi: 10.1080/21655979.2021.1960463

Figure Lengend Snippet: Effects of LRP2 knockdown on TG/lipid accumulation and lipogenesis in miR-380-5p -inhibited LO2 cells. (a) miR-380-5p expression level in LO2 cells cotransfected with the miR-380-5p inhibitor and si-LRP2 . (b) mRNA and (c and d) protein levels of SCD1, SREBP1 , and FAS in the miR-380-5p inhibitor- and si-LRP2 -cotransfected cells. (e) TG level and (f) lipid deposition in the miR-380-5p inhibitor- and si-LRP2 -cotransfected cells. *P < 0.05 vs. control or miR-380-5p mimic + si-NC

Article Snippet: Normal human hepatocyte lines LO2 were purchased from Biowit Biotechnology Inc. (cat no. C0009).

Techniques: Expressing